A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:00Preparation of Cells/Tissues
- 01:45Protein Extraction, Quality Control Western Blotting, In-solution Digestion, and Peptide Desalting
- 04:45TMT Labeling of Peptides
- 05:50Extensive High-resolution, Basic pH LC Prefactionation
- 06:43LC-MS/MS Preparation and Parameters
- 07:41Results: Deep Proteome Profiling Analysis
- 08:45Conclusion
