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应用实时 PCR 技术检测血清和血浆拷贝数
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Serum and Plasma Copy Number Detection Using Real-time PCR
Please note that all translations are automatically generated.
Click here for the English version.
应用实时 PCR 技术检测血清和血浆拷贝数
DOI:
10.3791/56502-v
•
09:21 min
•
December 15, 2017
•
Samanta Salvi
,
Vincenza Conteduca
,
Filippo Martignano
,
Giorgia Gurioli
,
Daniele Calistri
,
Valentina Casadio
1
Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS
Chapters
00:05
Title
00:55
Collection of Serum or Plasma
02:20
Isolating Cell-free (cfDNA) from Serum or Plasma
05:15
Real-time PCR and Analysis of cfDNA
07:36
Results: Detection of Androgen Receptor (AR) Copy Number Variations in cfDNA of Metastatic Castration-resistant Prostate Cancer (CRPC) Patients
08:25
Conclusion
Summary
Automatic Translation
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Automatic Translation
这篇手稿描述的拷贝数变异分析进行血清或血浆 DNA 使用 real-time PCR 方法。该方法适用于抗去势前列腺癌患者的耐药性预测, 但也可为其他疾病提供信息。
Tags
Serum
Plasma
Copy Number Detection
Real-time PCR
Cell-free DNA
Androgen Receptor
Gene Copy Number Variation
Metastatic Castration-resistant Prostate Cancer
Biomarker
Liquid Biopsy
Digital PCR
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