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Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death
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Immunology and Infection
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JoVE Journal Immunology and Infection
Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death

Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death

DOI:
10.3791/56535-v

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08:26 min

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October 31, 2017

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, , ,
1Department of Microbiology and Immunology,University of Louisville

Chapters

  • 00:05Title
  • 00:59Preparation of Mouse Bone Marrow Derived Macrophages (BMDMs) and BSA-fatty Acid Conjugate
  • 04:31Saturated Fatty Acids Induce Cell Death of Mouse Bone Marrow Derived Macrophages
  • 06:04Intracellular Ceramide Staining
  • 07:03Results: Fatty Acid-induced Macrophage Cell Death and Ceramide Production
  • 07:47Conclusion

Summary

Automatic Translation

Automatic Translation

We illustrate a straight-forward method to derive murine primary macrophages from bone marrow cells and a simple method to prepare BSA-fatty acid conjugates. Then we demonstrate that saturated fatty acids can induce macrophage cell death, and such cell death is positively associated with cellular accumulation of ceramide levels.

Tags

Keywords: Saturated Fatty AcidsMacrophagesCeramidesCell DeathMetabolic InflammationBSA Fatty Acid ConjugatesBone Marrow CellsRed Blood Cell LysisRPMI 1640 MediumMacrophage DifferentiationM-CSF

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