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Immunology and Infection

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JoVE Journal Immunology and Infection
Mättade fettsyror inducerar Ceramide-associerade makrofag celldöd
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Mättade fettsyror inducerar Ceramide-associerade makrofag celldöd

Article DOI: 10.3791/56535-v • 08:26 min • October 31st, 2017
October 31st, 2017 •
1, 1, 1, 1
1Department of Microbiology and Immunology, University of Louisville

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Vi illustrerar en okomplicerad metod för att härleda murina primära makrofager från benmärgsceller och en enkel metod att förbereda BSA-fettsyra konjugat. Då visar vi att mättade fettsyror kan inducera makrofag celldöd, och sådana celldöd är positivt associerad med cellulära ackumulering av ceramid nivåerna.

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Immunologi fråga 128 benmärg-derived makrofager BSA (bovint serum albumin) mättad fettsyra palmitinsyra stearinsyra ceramide celldöd
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