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December 21, 2017
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这篇手稿描述了一种方法, 标记个别信使 RNA (mRNA) 转录与荧光标记的 DNA 探针, 用于分子荧光原位杂交 (smFISH) 实验在大肠杆菌. smFISH 是一种可视化方法, 它允许在固定的单个细胞中同时检测、定位和量化单个 mRNA 分子。
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Cite this Article
Arbel-Goren, R., Shapira, Y., Stavans, J. Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments. J. Vis. Exp. (130), e56600, doi:10.3791/56600 (2017).
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