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Metode for mærkning udskrifter i individuelle Escherichia coli celler for enkelt-molekyle fluorescens In Situ hybridisering eksperimenter
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Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments

Metode for mærkning udskrifter i individuelle Escherichia coli celler for enkelt-molekyle fluorescens In Situ hybridisering eksperimenter

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8,069 Views

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07:51 min

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December 21, 2017

DOI:

10.3791/56600-v

DOI:
10.3791/56600-v

•

07:51 min
December 21, 2017

•

1 Views
, ,
1Department of Physics of Complex Systems,Weizmann Institute of Science

Summary

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Denne manuskriptet beskrives en metode til mærkning individuelle messenger RNA (mRNA) udskrifter med fluorescently mærket DNA-sonder, til brug i enkelt-molekyle fluorescens i situ hybridisering (smFISH) eksperimenter i E. coli. smFISH er en visualisering metode, der giver mulighed for samtidige påvisning, lokalisering og kvantificering af enkelt mRNA molekyler i faste individuelle celler.

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Arbel-Goren, R., Shapira, Y., Stavans, J. Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments. J. Vis. Exp. (130), e56600, doi:10.3791/56600 (2017).

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