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JoVE Journal Biology
Metod för märkning avskrifter i enskilda Escherichia coli celler för singel-molekyl fluorescens In Situ hybridisering experiment
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Metod för märkning avskrifter i enskilda Escherichia coli celler för singel-molekyl fluorescens In Situ hybridisering experiment

Article DOI: 10.3791/56600
December 21st, 2017 •
1, 1, 1
1Department of Physics of Complex Systems, Weizmann Institute of Science

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December 21st, 2017

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Click here for the English version.

Detta manuskript beskriver en metod för märkning enskilda budbärar-RNA (mRNA) avskrifter med fluorescently-märkt DNA sonder, för användning i singel-molekyl fluorescens i situ hybridisering (smFISH) experiment i E. coli. smFISH är en visualisering metod som tillåter den samtidiga upptäckt, lokalisering och kvantifiering av enstaka mRNA-molekyler i fast enskilda celler.

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