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Developmental Biology
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粘附细胞培养的时间推移荧光显微镜对蛋白质降解率的单细胞定量研究
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Developmental Biology
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JoVE Journal
Developmental Biology
Single-Cell Quantification of Protein Degradation Rates by Time-Lapse Fluorescence Microscopy in Adherent Cell Culture
Please note that all translations are automatically generated.
Click here for the English version.
粘附细胞培养的时间推移荧光显微镜对蛋白质降解率的单细胞定量研究
DOI:
10.3791/56604-v
•
12:06 min
•
February 04, 2018
•
Andrea Brigitta Alber
,
David Michael Suter
1
UPSUTER, Institute of Bioengineering (IBI), School of Life Sciences
,
École Polytechnique Fédérale de Lausanne (EPFL)
Chapters
00:00
Title
01:42
Cell seeding and pulse labeling of the SNAP-tag
04:15
Time-lapse microscopy
05:23
Image processing and analysis
07:55
Results
10:13
Conclusions
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一种方法, 以确定蛋白质半生命的单一生活粘附细胞, 使用脉冲标记和荧光时间推移成像的捕捉标签融合蛋白。
Tags
Protein Degradation
Single-cell
Time-lapse Fluorescence Microscopy
Adherent Cell Culture
SNAP-tag
Protein Half-life
Fluorescence Imaging
Protein Turnover
Heterogeneity
Endogenous Protein Tagging
Overexpression
DNA Repair Enzyme
Benzylguanine
Fluorescent Dye
Exponential Decay
Embryonic Stem Cells
Monolayer
Cell-cell Interactions
N-cadherin
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