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一种有效的
体外
转位方法由转录调节的睡眠美容系统封装到一个集成缺陷的慢向量
JoVE Journal
Biology
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JoVE Journal
Biology
An Efficient
In Vitro
Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector
Please note that all translations are automatically generated.
Click here for the English version.
一种有效的
体外
转位方法由转录调节的睡眠美容系统封装到一个集成缺陷的慢向量
DOI:
10.3791/56742-v
•
10:13 min
•
January 12, 2018
•
Daniela Benati
,
Fabienne Cocchiarella
,
Alessandra Recchia
1
Centre for Regenerative Medicine, Department of Life Sciences
,
University of Modena and Reggio Emilia
Chapters
00:05
Title
00:51
Transduction of Human Primary Keratinocytes
04:31
Cytofluorimetric Analysis of Transduced Primary Keratinocytes
06:24
Quantitative RT-PCR (qRT-PCR)
07:24
Results: Sleeping Beauty System Achieves Stable Integration of a GOI in the Human Genome
08:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议描述了一种方法, 以实现稳定的基因的兴趣进入人类基因组由转录调控的
睡美人
系统。本文报道了有缺陷的慢载体的合成、人体细胞的
体外
转导和转细胞的分子检测。
Tags
Sleeping Beauty System
Integration Defective Lentiviral Vector
Gene Therapy
Primary Cells
Transcriptionally Regulated
Transposition Method
In Vitro
Hyperactive SB100X Transposase
Gene Of Interest
Tetracycline-regulated
Polybrene
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