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Isolation, kultur og differentiering af knoglemarven stromale celler og osteoklast ophav fra mus
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
DOI:

08:07 min

January 06, 2018

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Chapters

  • 00:05Title
  • 00:41Collection Tube Preparation and Bone Harvest
  • 02:52Total Bone Marrow Cell (BMC) and Split BMC Population Plating and Culture
  • 04:45Bone Marrow Stem Cell (BMSC) Differentiation
  • 06:07Results: Representative BMSC Differentiation
  • 07:27Conclusion

Summary

Automatic Translation

I denne artikel præsenterer vi metoder for at isolere og differentiere knoglemarv stromale celler og hæmatopoietisk stamceller fra mus lange knogler. To forskellige protokoller præsenteres højtydende forskellige cellepopulationer egnet til udvidelse og differentiering af osteoblaster, adipocytter og osteoklaster.

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