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多肽基基质辅助激光解吸/电离质谱成像中福尔马林固定样品的优化制备工作流
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows
DOI:

08:16 min

January 16, 2018

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Chapters

  • 00:05Title
  • 00:29Preparation of Reagents and Equipment
  • 01:21Preparation of Tissue Sections and Methylene Crosslink Hydrolysis
  • 02:40Tissue Digestion
  • 03:57Matrix Coating via Sublimation
  • 05:26Recrystallization and Instrumentation
  • 06:20Results: Correctly and Incorrectly Prepared Human Brain Tissue
  • 07:07Conclusion

Summary

Automatic Translation

本协议描述了一种可重现且可靠的方法, 用于 sublimation-based 制备用于成像质谱的福尔马林固定组织。

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