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Journal
/
Biochemistry
/
多肽基基质辅助激光解吸/电离质谱成像中福尔马林固定样品的优化制备工作流
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows
Please note that all translations are automatically generated.
Click here for the English version.
多肽基基质辅助激光解吸/电离质谱成像中福尔马林固定样品的优化制备工作流
DOI:
10.3791/56778-v
•
08:16 min
•
January 16, 2018
•
Matthew B. O’Rourke
2
,
Matthew P. Padula
,
Caine Smith
,
Priscilla Youssef
,
Stuart Cordwell
,
Paul Witting
,
Greg Sutherland
,
Ben Crossett
1
Mass Spectrometry Core Facility
,
University of Sydney
,
2
Proteomics Core Facility
,
University of Technology Sydney
,
3
Neuropathology Group, Discipline of Pathology, School of Medical Sciences
,
University of Sydney
,
4
Redox Biology Group, Discipline of Pathology, School of Medical Sciences
,
University of Sydney
Chapters
00:05
Title
00:29
Preparation of Reagents and Equipment
01:21
Preparation of Tissue Sections and Methylene Crosslink Hydrolysis
02:40
Tissue Digestion
03:57
Matrix Coating via Sublimation
05:26
Recrystallization and Instrumentation
06:20
Results: Correctly and Incorrectly Prepared Human Brain Tissue
07:07
Conclusion
Summary
Automatic Translation
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Automatic Translation
本协议描述了一种可重现且可靠的方法, 用于 sublimation-based 制备用于成像质谱的福尔马林固定组织。
Tags
Formalin Fixed
Peptide Mass Spectrometry Imaging
Nitrocellulose Coating
Vapor Chamber
Xylene
Ethanol
Carnoy’s Fluid
Tris Buffer
Pressure Cooker
Trypsin
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