/
/
Optimale Vorbereitung von Formalin fixiert Proben für Peptid-Matrix basierten Assisted Laser Desorption/Ionisierung Massenspektrometrie Imaging-Workflows
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Mass Spectrometry Core Facility,University of Sydney, 2Proteomics Core Facility,University of Technology Sydney, 3Neuropathology Group, Discipline of Pathology, School of Medical Sciences,University of Sydney, 4Redox Biology Group, Discipline of Pathology, School of Medical Sciences,University of Sydney
Chapters
- 00:05Title
- 00:29Preparation of Reagents and Equipment
- 01:21Preparation of Tissue Sections and Methylene Crosslink Hydrolysis
- 02:40Tissue Digestion
- 03:57Matrix Coating via Sublimation
- 05:26Recrystallization and Instrumentation
- 06:20Results: Correctly and Incorrectly Prepared Human Brain Tissue
- 07:07Conclusion
