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Optimale Vorbereitung von Formalin fixiert Proben für Peptid-Matrix basierten Assisted Laser Desorption/Ionisierung Massenspektrometrie Imaging-Workflows
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows
DOI:

08:16 min

January 16, 2018

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Chapters

  • 00:05Title
  • 00:29Preparation of Reagents and Equipment
  • 01:21Preparation of Tissue Sections and Methylene Crosslink Hydrolysis
  • 02:40Tissue Digestion
  • 03:57Matrix Coating via Sublimation
  • 05:26Recrystallization and Instrumentation
  • 06:20Results: Correctly and Incorrectly Prepared Human Brain Tissue
  • 07:07Conclusion

Summary

Automatic Translation

Dieses Protokoll beschreibt eine reproduzierbare und zuverlässige Methode für die Sublimation-basierte Erstellung von Formalin fixiert Gewebe für bildgebende Massenspektrometrie bestimmt.

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