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Developmental Biology
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单细胞指数分型与内皮细胞小生境联合培养对胚胎造血干细胞前体的克隆分析
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture
Please note that all translations are automatically generated.
Click here for the English version.
单细胞指数分型与内皮细胞小生境联合培养对胚胎造血干细胞前体的克隆分析
DOI:
10.3791/56973-v
•
09:32 min
•
May 08, 2018
•
Brandon K. Hadland
2
,
Barbara Varnum-Finney
,
Cynthia Nourigat-Mckay
,
David Flowers
,
Irwin D. Bernstein
2
1
Clinical Research Division
,
Fred Hutchinson Cancer Research Center
,
2
Department of Pediatrics, Division of Pediatric Hematology/Oncology
,
University of Washington School of Medicine
Chapters
00:05
Title
00:52
Murine Embryonic Cell Staining
02:49
Single Hemogenic Precursor Index Sorting
04:44
Clonal Hematopoietic Progeny and Individual Clone Engraftment Property Analyses
07:02
Results: Representative Embryonic Hematopoietic Stem Cell Precursor Analysis
09:08
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文介绍了小鼠胚胎发育过程中造血干细胞前体的克隆分析方法。我们结合内皮细胞共培养和移植对胚胎主动脉-性腺-中区单细胞的指标进行分类, 以表征单造血前体的表型性质和植入电位。
Tags
Clonal Analysis
Embryonic Hematopoietic Stem Cell Precursors
Single Cell Index Sorting
Endothelial Cell Niche Co-culture
Developmental Hematopoiesis
Lineage Contributions
Hemogenic Precursors
Phenotypic Properties
HSC Precursors
Collagenase
Antibody Cocktail
Flow Cytometry
Single Cell Sorting
Index Sorting
Article
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