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Journal
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Biochemistry
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精製マウス肝類洞内皮細胞と肝細胞の灌流
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Purification of Hepatocytes and Sinusoidal Endothelial Cells from Mouse Liver Perfusion
Please note that all translations are automatically generated.
Click here for the English version.
精製マウス肝類洞内皮細胞と肝細胞の灌流
DOI:
10.3791/56993-v
•
08:54 min
•
February 12, 2018
•
Fatima Cabral
,
Colton M. Miller
,
Katrina M. Kudrna
,
Blake E. Hass
,
Jocelyn G. Daubendiek
,
Brianna M. Kellar
,
Edward N. Harris
1
Department of Biochemistry
,
University of Nebraska
Chapters
00:04
Title
00:45
Catheter Placement
02:46
Liver Perfusion and Hepatocyte Purification
04:39
Sinusoidal Endothelial Cell (SEC) Purification
07:18
Results: Representative SEC Purification Analyses
08:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
このプロトコルの目的は、肝臓から高生存率および肝細胞と類洞内皮細胞の高収率を取得することです。これは、灌肝門脈、肝細胞と類洞内皮細胞を取得する差動遠心分離の続くタイプ IV コラゲナーゼの解決で。
Tags
Mouse Liver Perfusion
Hepatocyte Purification
Sinusoidal Endothelial Cell Purification
Liver Cell Populations
Portal Vein Catheterization
Liver Digestion
Liver Cell Viability
Hepatocyte Harvesting
Buffer Solutions
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