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Developmental Biology
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用于在大容量中查找特定细胞目标的串行剖面的多模式分层成像
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Multimodal Hierarchical Imaging of Serial Sections for Finding Specific Cellular Targets within Large Volumes
Please note that all translations are automatically generated.
Click here for the English version.
用于在大容量中查找特定细胞目标的串行剖面的多模式分层成像
DOI:
10.3791/57059-v
•
11:19 min
•
March 20, 2018
•
Irene U. Wacker
2
,
Lisa Veith
,
Waldemar Spomer
4
,
Andreas Hofmann
4
,
Marlene Thaler
,
Stefan Hillmer
,
Ulrich Gengenbach
4
,
Rasmus R. Schröder
2,3
1
Cryo Electron Microscopy, Centre for Advanced Materials
,
Universität Heidelberg
,
2
Heidelberg Karlsruhe Research Partnership (HEiKA)
,
3
Cryo Electron Microscopy, BioQuant
,
Universitätsklinikum Heidelberg
,
4
Institute for Automation and Applied Computer Science
,
Karlsruhe Institute of Technology (KIT)
,
5
Carl Zeiss Microscopy GmbH
,
6
Electron Microscopy Core Facility
,
Universität Heidelberg
Chapters
00:04
Title
01:16
Ultramicrotome and Sample Setup
03:04
Ultramicrotome Sectioning
04:21
Hierarchical Imaging in the SEM
07:33
Registration of the SEM Image Stack
08:39
Results: Multimodal Hierarchical Imaging of Serial Sections of Plant Roots
10:06
Conclusion
Summary
Automatic Translation
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Automatic Translation
本协议以扫描电镜 (SEM) 为目标, 以纳米尺度分辨率成像组织中的特定细胞为靶点。从树脂嵌入的生物材料中, 大量的系列部分首先在光学显微镜中被成像, 以识别目标, 然后以分层的方式在 SEM 中。
Tags
Multimodal Imaging
Serial Sections
Cellular Targets
Ultrastructure
Array Tomography
Light Microscopy
SEM
Cell Biology
Developmental Biology
Neuroscience
Pathology
Silicon Wafer
Substrate Preparation
Diamond Knife
Clearance Angle
Knife Boat
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