13,713 Views
•
11:23 min
•
January 09, 2018
DOI:
Transcript
Automatically generated
Summary
Automatically generated
Здесь мы представляем протокол анализировать состав протеина большой родной: протеин и белка: комплексов нуклеиновых кислот из рапса (B. napus) флоэма экссудата с использованием 3D геля полиакриламида электрофореза (страница) подхода, сочетающего синий родной (BN) с двумя денатурируя страниц следуют масс-спектрометрических идентификации.
Related Videos
12:29
mRNA Interactome Capture from Plant Protoplasts
Related Videos
9037 Views
08:23
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
Related Videos
11261 Views
08:26
Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
Related Videos
6554 Views
09:33
High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
Related Videos
7147 Views
05:47
Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
Related Videos
5116 Views
10:10
Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana
Related Videos
15748 Views
08:21
Detection of Protein Interactions in Plant using a Gateway Compatible Bimolecular Fluorescence Complementation (BiFC) System
Related Videos
25277 Views
12:07
Profiling Thiol Redox Proteome Using Isotope Tagging Mass Spectrometry
Related Videos
16161 Views
09:38
Collection and Analysis of Arabidopsis Phloem Exudates Using the EDTA-facilitated Method
Related Videos
24369 Views
10:26
The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example
Related Videos
36850 Views
Read Article
Cite this Article
Pahlow, S., Ostendorp, A., Krüßel, L., Kehr, J. Phloem Sap Sampling from Brassica napus for 3D-PAGE of Protein and Ribonucleoprotein Complexes. J. Vis. Exp. (131), e57097, doi:10.3791/57097 (2018).
Copy