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Bioengineering
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凝胶序列: 一种同时测序库的方法用水凝胶基质制备 DNA 和 RNA
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices
Please note that all translations are automatically generated.
Click here for the English version.
凝胶序列: 一种同时测序库的方法用水凝胶基质制备 DNA 和 RNA
DOI:
10.3791/57315-v
•
09:19 min
•
March 26, 2018
•
Gordon D. Hoople*
1
,
Andrew Richards*
2
,
Yan Wu
,
Albert P. Pisano
,
Kun Zhang
1
Department of Engineering
,
University of San Diego
,
2
Department of Bioengineering
,
University of California, San Diego
,
3
Department of Mechanical and Aerospace Engineering
,
University of California, San Diego
Chapters
00:04
Title
00:42
Gel-seq Cassette Fabrication
03:12
Gel Separation
04:36
Sample Recovery
06:53
Results: Gel-seq Compared with Conventional Methods
08:26
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
凝胶序列使研究人员能够同时为 DNA 和 RNA 的存储提供从 100-1000 细胞开始使用简单的水凝胶装置的可忽略的额外成本。本文给出了该装置的制作方法, 以及生成配对库的生物协议。
Tags
Gel-seq
Simultaneous Sequencing
DNA And RNA
Hydrogel Matrices
Next-generation Sequencing
Genomic And Transcriptomic Information
Filler Gel
High Density Gel
Low Density Gel
Gel Electrophoresis Cassette
Polymer Curing
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