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09:38 min
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March 28, 2018
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Deux différentes 3' amplification rapide des extrémités d’ADNc (3' course) protocoles décrit ici faire usage de deux différents ADN polymérases pour mapper les séquences qui incluent une partie de la trame de lecture ouverte (ORF), le codon d’arrêt et l’entière région 3' UTR de transcription utilisant l’ARN issus de lignées de cellules cancéreuses différent.
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Cite this Article
Masamha, C. P., Todd, Z. Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer. J. Vis. Exp. (133), e57318, doi:10.3791/57318 (2018).
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