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JoVE Journal Genetics
Adattamento 3' amplificazione veloce di CDNA finisce per mappare le trascrizioni in cancro
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Determining the Likelihood of Variant Pathogenicity Using Amino Acid-level Signal-to-Noise Analysis of Genetic Variation
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Determining the Likelihood of Variant Pathogenicity Using Amino Acid-level Signal-to-Noise Analysis of Genetic Variation
 
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Adattamento 3' amplificazione veloce di CDNA finisce per mappare le trascrizioni in cancro

Article DOI: 10.3791/57318
March 28th, 2018 •
1, 1
1Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Butler University

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March 28th, 2018

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I due diversi 3' amplificazione veloce delle estremità del cDNA (3' gara) protocolli descritto qui fanno uso di due diversi DNA polimerasi per mappare sequenze che includono un segmento dell'open reading frame (ORF), il codone di arresto e l'intero regione 3' UTR di una trascrizione usando RNA ottenute da linee cellulari tumorali differenti.

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