Journal
/
/
Udvikling og validering af et Ultrasensitive enkelt molekyle Array Digital enzym-forbundet Immunosorbent Assay for menneskelige Interferon-α
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
DOI:

08:26 min

June 14, 2018

, , , , ,

Chapters

  • 00:05Title
  • 01:03Paramagnetic Bead Preparation, Activation, and Antibody-conjugation
  • 03:26Bead Blocking and Final Clean-up
  • 04:50Assay Optimization
  • 07:06Results: Application of an Optimized IFN-α Assay
  • 07:40Conclusion

Summary

Automatic Translation

Her præsenterer vi en protokol til at beskrive udviklingen og valideringen af et enkelt molekyle array digital ELISA assay, som gør det ultra-følsomme påvisning af alle IFN-α undertyper i menneskelige prøver.

Related Videos

Read Article