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Immunology and Infection
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Udvikling og validering af et Ultrasensitive enkelt molekyle Array Digital enzym-forbundet Immunosorbent Assay for menneskelige Interferon-α
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Udvikling og validering af et Ultrasensitive enkelt molekyle Array Digital enzym-forbundet Immunosorbent Assay for menneskelige Interferon-α

DOI:
10.3791/57421-v

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08:26 min

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June 14, 2018

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, , , , ,
1Immunobiology of Dendritic Cells,Institut Pasteur, 2INSERM U1223, 3Laboratory of Neurogenetics and Neuroinflammation, INSERM UMR1163,Institut Imagine, 4MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine,University of Edinburgh, 5Manchester Centre for Genomic Medicine,University of Manchester

Chapters

  • 00:05Title
  • 01:03Paramagnetic Bead Preparation, Activation, and Antibody-conjugation
  • 03:26Bead Blocking and Final Clean-up
  • 04:50Assay Optimization
  • 07:06Results: Application of an Optimized IFN-α Assay
  • 07:40Conclusion

Summary

Automatic Translation

Automatic Translation

Her præsenterer vi en protokol til at beskrive udviklingen og valideringen af et enkelt molekyle array digital ELISA assay, som gør det ultra-følsomme påvisning af alle IFN-α undertyper i menneskelige prøver.

Tags

Single Molecule ArrayDigital ELISAInterferon-γUltrasensitiveBiomarker DiscoveryAutoimmune Polyendocrine Syndrome Type 1Paramagnetic BeadsEDCAntibody Conjugation

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