This content is Free Access.
1Department of Dermatology, Program in Epithelial Biology,Stanford University School of Medicine, 2Program in Cancer Biology,Stanford University School of Medicine, 3Canary Center for Cancer Early Detection, Department of Radiology,Stanford University School of Medicine, 4Department of Biology,Bridgewater State University, 5System Biosciences, 6Veterans Affairs Healthcare System
Chapters
- 00:04Title
- 01:02Plasmid Preparation and gRNA Insertion
- 03:26Lentivirus Production
- 05:12Lentivirus Transduction of Cells / Cell Dimerization
- 07:08Results: CLOuD9 Induces Reversible β-Globin Promoter-LCR Looping
- 08:55Conclusion
Kromatin looping spiller en væsentlig rolle i genregulering; der har dog været nogen teknologiske fremskridt, som giver mulighed for selektiv og reversible ændring af kromatin sløjfer. Her beskriver vi et kraftfuldt system for kromatin loop re organisation ved hjælp af CRISPR-dCas9 (CLOuD9), viste at selektivt og reversibelt modulere genekspression på målrettet loci.
