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La determinazione della specificità della proteasi nel Mouse tessuto estratti mediante spettrometria di massa MALDI-TOF: manipolazione di PH per causare i cambiamenti di specificità
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes
DOI:

09:47 min

May 25, 2018

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Chapters

  • 00:05Title
  • 00:36Preparation of Tissue Extracts
  • 01:40Digestion of Substrates in Model Protease and Tissue Extracts
  • 02:59Purification of the Iminobiotin-labeled Substrate
  • 05:17Sample Preparation and Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF Mass Spectrometry)
  • 06:48Results: Identification of Protease Specificity
  • 08:46Conclusion

Summary

Automatic Translation

Qui, presentiamo un protocollo per determinare la specificità di proteasi in tessuto grezzo del mouse estratti usando spettrometria MALDI-TOF.

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