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Evaluering af virkningen af Protein aggregering på cellulært Oxidative Stress i gær
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast
DOI:

11:04 min

June 23, 2018

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Chapters

  • 00:05Title
  • 01:10S. cerevisiae Cultures and Protein Expression
  • 03:16Cell Staining
  • 04:29Flow Cytometry Analysis
  • 09:05Results: Protein Inclusions Play a Protective Role Against Oxidative Stress
  • 10:38Conclusion

Summary

Automatic Translation

Protein sammenlægning fremkalder cellulære oxidativt stress. Denne protokol beskriver en metode til overvågning af de intracellulære stater af amyloidogenic proteiner og den oxidative stress i forbindelse med dem, ved hjælp af flowcytometri. Metoden bruges til at studere funktionen af opløselige og sammenlægning-tilbøjelige varianter af amyloid-β-peptid.

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