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成人脑组织切片中钾离子选择性电极的制备、检测及应用

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain

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成人脑组织切片中钾离子选择性电极的制备、检测及应用

DOI:

10.3791/57511-v

•

11:20 min

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May 07, 2018

•

J. Christopher Octeau, Guido Faas, Istvan Mody², Baljit S. Khakh³

¹Department of Physiology, David Geffen School of Medicine,University of California Los Angeles, ²Department of Neurology, David Geffen School of Medicine,University of California Los Angeles, ³Department of Neurobiology, David Geffen School of Medicine,University of California Los Angeles

Chapters

00:14Title
00:46Preparation of Potassium Ion-selective Microelectrodes
04:18Calibration of Potassium Ion-selective Microelectrodes
05:40Acute Slice Preparation
07:16Measurement of Electrically Evoked K Release
09:26Results: Calibration of Microelectrodes and Measurement of Electrically Evoked K Release
10:53Conclusion

Summary

Automatic Translation

钾离子有助于细胞的静止膜电位, 胞外 K⁺浓度是细胞兴奋性的重要调节因子。我们描述如何制作, 校准和使用单 K⁺选择性电极。使用此类电极可以测量成年海马切片中电诱发的 K⁺浓度动态。

Tags

Keywords: Potassium Ion Selective Microelectrodes Adult Brain Potassium Ion Homeostasis Borosilicate Glass Capillaries Silanization Microelectrode Fabrication DDS Silanization Solution Microelectrode Puller Potassium Ion Concentration Measurement

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