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Neuroscience
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成人脑组织切片中钾离子选择性电极的制备、检测及应用
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain
Please note that all translations are automatically generated.
Click here for the English version.
成人脑组织切片中钾离子选择性电极的制备、检测及应用
DOI:
10.3791/57511-v
•
11:20 min
•
May 07, 2018
•
J. Christopher Octeau
,
Guido Faas
,
Istvan Mody
2
,
Baljit S. Khakh
3
1
Department of Physiology, David Geffen School of Medicine
,
University of California Los Angeles
,
2
Department of Neurology, David Geffen School of Medicine
,
University of California Los Angeles
,
3
Department of Neurobiology, David Geffen School of Medicine
,
University of California Los Angeles
Chapters
00:14
Title
00:46
Preparation of Potassium Ion-selective Microelectrodes
04:18
Calibration of Potassium Ion-selective Microelectrodes
05:40
Acute Slice Preparation
07:16
Measurement of Electrically Evoked K
Release
09:26
Results: Calibration of Microelectrodes and Measurement of Electrically Evoked K
Release
10:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
钾离子有助于细胞的静止膜电位, 胞外 K
+
浓度是细胞兴奋性的重要调节因子。我们描述如何制作, 校准和使用单 K
+
选择性电极。使用此类电极可以测量成年海马切片中电诱发的 K
+
浓度动态。
Tags
Potassium Ion Selective Microelectrodes
Adult Brain
Potassium Ion Homeostasis
Borosilicate Glass Capillaries
Silanization
Microelectrode Fabrication
DDS Silanization Solution
Microelectrode Puller
Potassium Ion Concentration Measurement
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