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Virus levering af CRISPR Guides til Murine prostata for gen ændring
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Virus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration
DOI:

06:21 min

April 27, 2018

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Chapters

  • 00:04Title
  • 00:47Surgical Preparations
  • 01:40Virus Delivery to the Murine Prostate
  • 04:35Results: Loss of Pten Expression in the Prostate Using CRISPR/Cas9 Gene Editing
  • 05:45Conclusion

Summary

Automatic Translation

Denne protokol beskriver en nyetableret metode til virus levering til murine prostata. Bruger CRISPR/Cas9 teknologi, gen overekspression eller Cre recombinase levering, teknikken tillader orthotopic ændring af genekspression og implementerer en roman musemodel for prostatakræft.

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