A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:04Title
- 00:47Surgical Preparations
- 01:40Virus Delivery to the Murine Prostate
- 04:35Results: Loss of Pten Expression in the Prostate Using CRISPR/Cas9 Gene Editing
- 05:45Conclusion
Denne protokol beskriver en nyetableret metode til virus levering til murine prostata. Bruger CRISPR/Cas9 teknologi, gen overekspression eller Cre recombinase levering, teknikken tillader orthotopic ændring af genekspression og implementerer en roman musemodel for prostatakræft.
