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转化生长因子β信号诱导内皮-间质转移的分子分析
JoVE Journal
Biology
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JoVE Journal Biology
Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
DOI:

07:49 min

August 03, 2018

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Chapters

  • 00:04Title
  • 00:41Induction of EndMT
  • 01:58Immunocytochemical Analysis
  • 05:02Three-dimensional Collagen Gel Contraction Assay
  • 06:09Results: Use of an In Vitro Method to Study Endothelial-mesenchymal Transition Upon TGF-β Treatment
  • 07:20Conclusion

Summary

Automatic Translation

本文介绍了一种体外诱导内皮-间质转移 (EndMT) 的方法, 该协议对于研究 EndMT 中涉及的细胞信号通路是有用的。在该实验模型中, EndMT 是由 TGF β在 MS-1 内皮细胞中的治疗引起的。

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