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Bioengineering
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单细胞基因组测序的超高通量微流控平台
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
Please note that all translations are automatically generated.
Click here for the English version.
单细胞基因组测序的超高通量微流控平台
DOI:
10.3791/57598-v
•
10:00 min
•
May 23, 2018
•
Benjamin Demaree
2
,
Daniel Weisgerber
,
Freeman Lan
2
,
Adam R. Abate
2,3
1
Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences
,
University of California, San Francisco
,
2
UC Berkeley-UCSF Graduate Program in Bioengineering
,
University of California, San Francisco
,
3
Chan Zuckerberg Biohub
Chapters
00:05
Title
00:48
Encapsulation of Cells in Agarose Microgels
02:48
Breaking the Agarose Microgels
03:54
Generating Barcode Droplets by Digital PCR
04:53
Tagmentation of Genomic DNA in Droplets
05:48
Single-cell Barcoding by Microfluidic Double Merger
08:25
Results: Ultrahigh Throughput Sequencing of Single-cell Genomes
09:24
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
单细胞测序揭示了生物系统中的基因型异质性, 但目前的技术缺乏对群落组成和功能的深入分析所必需的吞吐量。在这里, 我们描述了一个微流控流程的序列 > 5万单细胞基因组从不同的细胞群。
Tags
Single-cell
Genome Sequencing
Microfluidics
High-throughput
Barcoding
Cell Lysis
Agarose
Droplet Generation
Cell Encapsulation
Genomic Heterogeneity
Cell Suspension
PBS
Surfactant
Syringe Pump
Temperature Control
Collection
Oil Removal
PFO
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