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Cancer Research
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基于免疫荧光显微镜的瞬时和持久双链 dna 损伤 dna 修复过程的表征
JoVE Journal
Cancer Research
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JoVE Journal
Cancer Research
Characterizing DNA Repair Processes at Transient and Long-lasting Double-strand DNA Breaks by Immunofluorescence Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
基于免疫荧光显微镜的瞬时和持久双链 dna 损伤 dna 修复过程的表征
DOI:
10.3791/57653-v
•
08:31 min
•
June 08, 2018
•
Vaibhav Murthy*
1
,
Dalton Dacus*
1
,
Monica Gamez
,
Changkun Hu
,
Sebastian O. Wendel
,
Jazmine Snow
,
Andrew Kahn
,
Stephen H. Walterhouse
,
Nicholas A. Wallace
1
Division of Biology
,
Kansas State University
,
2
Bristol Medical School, Translational Health Sciences
,
University of Bristol
Chapters
00:40
Defining the Kinetics of DSB Repair Complex Formation
03:38
Defining Nuclei in Immunofluorescence Images
05:44
Analyzing Localization to a Long-lasting Double-strand DNA Break
06:40
Results: Co-localization of Double-strand DNA Breaks and Repair Proteins by Immunofluorescence Microscopy
07:46
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
双链 DNA 断裂的修复是一个动态过程, 不仅需要在断裂时形成修复配合物, 而且要解决病变后的解决方法。在这里, 我们使用免疫荧光显微镜的短暂和长期的双滞留断裂作为一个工具来解剖这个基因组维持机制。
Tags
DNA Repair
Double-strand DNA Breaks
Immunofluorescence Microscopy
DNA Repair Complex
U2OS/DR-GFP Cells
EDTA
Trypsin
DMEM
Hydrogen Peroxide
PBS
PFA
Non-ionic Detergent
BSA
Primary Antibodies
DAPI
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