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肿瘤免疫和非免疫微环境在小鼠皮下肿瘤中的富集与鉴定
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Cancer Research
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JoVE Journal Cancer Research
Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
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肿瘤免疫和非免疫微环境在小鼠皮下肿瘤中的富集与鉴定

DOI:
10.3791/57685-v

•

08:32 min

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June 07, 2018

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, ,
1Department of Otolaryngology-Head and Neck Surgery,Baylor College of Medicine, 2Interdepartmental Graduate Program in Translational Biology and Molecular Medicine,Baylor College of Medicine

Chapters

  • 00:04Title
  • 00:55Tumor Harvest and Digestion
  • 03:02Immune and Tumor Cellular Fraction Separation and Labeling
  • 05:57Results: Representative Tumor Component Features
  • 07:27Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们描述了一个方法, 以分离和丰富的组成部分, 肿瘤免疫和非免疫微环境的建立皮下肿瘤。该技术允许对肿瘤免疫浸润和非免疫肿瘤分数进行单独分析, 从而可以对肿瘤免疫微环境进行综合表征。

Tags

Tumor Immune MicroenvironmentTumor Non-immune MicroenvironmentImmune OncologyTumor ProfilingTumor DissociationTumor EnrichmentTumor Cell FractionationTumor Immune Cell SeparationCollagenaseDNaseDensity Gradient MediumSubcutaneous Tumors

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