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Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton
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Developmental Biology
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JoVE Journal Developmental Biology
Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

DOI:
10.3791/57702-v

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08:01 min

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April 19, 2018

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,
1Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Department of Anatomy and Developmental Biology,Monash University

Chapters

  • 00:05Title
  • 00:41Nuclei Number and Distribution Post Imaging Analysis
  • 03:58Sperm and Chromosome Number Scoring and Cytoskeletal Germline Reconstruction Post Imaging Analyses
  • 05:42Results: Representative Germline Imaging Analyses
  • 07:20Conclusion

Summary

Automatic Translation

Automatic Translation

We present an automated method for three-dimensional reconstruction of the Caenorhabditis elegans germline. Our method determines the number and position of each nucleus within the germline and analyses germline protein distribution and cytoskeletal structure.

Tags

Caenorhabditis ElegansGermlineNucleiProteinsCytoskeletonComputational AnalysisImage AnalysisConfocal MicroscopyMitotic RegionTransition ZoneDAPISurface FunctionSpot Function3D ModelingImage AcquisitionImage Processing

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