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秀丽线虫生殖的计算分析研究细胞核、蛋白质和细胞骨架的分布
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Developmental Biology
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JoVE Journal Developmental Biology
Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton
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秀丽线虫生殖的计算分析研究细胞核、蛋白质和细胞骨架的分布

DOI:
10.3791/57702-v

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08:01 min

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April 19, 2018

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,
1Development and Stem Cells Program, Monash Biomedicine Discovery Institute, Department of Anatomy and Developmental Biology,Monash University

Chapters

  • 00:05Title
  • 00:41Nuclei Number and Distribution Post Imaging Analysis
  • 03:58Sperm and Chromosome Number Scoring and Cytoskeletal Germline Reconstruction Post Imaging Analyses
  • 05:42Results: Representative Germline Imaging Analyses
  • 07:20Conclusion

Summary

Automatic Translation

Automatic Translation

我们为秀丽线虫生殖提供三维重建的自动方法.我们的方法确定生殖中每个核的数量和位置, 并分析生殖蛋白质分布和骨架结构。

Tags

Caenorhabditis ElegansGermlineNucleiProteinsCytoskeletonComputational AnalysisImage AnalysisConfocal MicroscopyMitotic RegionTransition ZoneDAPISurface FunctionSpot Function3D ModelingImage AcquisitionImage Processing

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