JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
小鼠原发性肾管上皮细胞的分离、鉴定及高通量的细胞外流量分析
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells
Please note that all translations are automatically generated.
Click here for the English version.
小鼠原发性肾管上皮细胞的分离、鉴定及高通量的细胞外流量分析
DOI:
10.3791/57718-v
•
09:40 min
•
June 20, 2018
•
Wen Ding
2
,
Keyvan Yousefi
2
,
Lina A. Shehadeh
3,4,5
1
Department of Molecular and Cellular Pharmacology
,
University of Miami Leonard M. Miller School of Medicine
,
2
Interdisciplinary Stem Cell Institute
,
University of Miami Leonard M. Miller School of Medicine
,
3
Department of Medicine, Division of Cardiology
,
University of Miami Leonard M. Miller School of Medicine
,
4
Vascular Biology Institute
,
University of Miami Leonard M. Miller School of Medicine
,
5
Peggy and Harold Katz Family Drug Discovery Center
,
University of Miami Leonard M. Miller School of Medicine
Chapters
00:04
Title
00:50
Preparing Collagen I Plates
01:31
Perfusion, Digestion and Harvesting Kidneys from Mice
02:36
Tissue Processing and Primary Tubular Cells Isolation
04:11
Mitochondria Bioenergetics Assay
07:26
Results: Renal Tubular Epithelial Cell (TEC) Morphology and Metabolism
09:04
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
该协议提供了一种经济高效的方法, 以分离和表征小鼠原发性肾管细胞, 随后可以进行亚培养, 以评估
肾脏生物学功能, 包括线粒体生物能学。
Tags
Renal Metabolism
Fatty Acid Oxidation
Renal Fibrosis
Nephropathies
Mitochondrial Bioenergetics
Renal Epithelial Cells
High-throughput Screening
Renal Metabolic Disease
Primary Renal Tubular Epithelial Cells
Collagen-coated Dishes
Mouse Kidney Perfusion
Kidney Digestion
Tubular Cell Isolation
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
一个神经元和星形胶质细胞共培养检测神经毒性的高含量分析
分离小鼠原代滋养细胞及滋养细胞侵袭实验
诱导上皮和分析间质转化
小鼠原代胰腺腺泡细胞的分离和培养
维甲酸的应用获取从初级小鼠成骨细胞骨细胞培养
隔离和鼠标主动脉内皮细胞原代培养
细胞外通量分析技术在视网膜组织外种中的能量代谢测定
原发性小鼠视网膜色素上皮细胞的有效解剖与培养
使用细胞外通量分析仪评估小鼠造血干细胞和原始祖细胞中的细胞生物能量学
小鼠原发性肝正弦内皮细胞的分离与表征
Read Article