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1Department of Molecular and Cellular Pharmacology,University of Miami Leonard M. Miller School of Medicine, 2Interdisciplinary Stem Cell Institute,University of Miami Leonard M. Miller School of Medicine, 3Department of Medicine, Division of Cardiology,University of Miami Leonard M. Miller School of Medicine, 4Vascular Biology Institute,University of Miami Leonard M. Miller School of Medicine, 5Peggy and Harold Katz Family Drug Discovery Center,University of Miami Leonard M. Miller School of Medicine
Chapters
- 00:04Title
- 00:50Preparing Collagen I Plates
- 01:31Perfusion, Digestion and Harvesting Kidneys from Mice
- 02:36Tissue Processing and Primary Tubular Cells Isolation
- 04:11Mitochondria Bioenergetics Assay
- 07:26Results: Renal Tubular Epithelial Cell (TEC) Morphology and Metabolism
- 09:04Conclusion
Dieses Protokoll bietet eine kostengünstige Möglichkeit zu isolieren und zu charakterisieren Maus primäre renale tubuläre Zellen, die anschließend südlich kultiviert werden können, um renale biologischen Funktionen ex Vivo, einschließlich der mitochondrialen Bioenergetik zu beurteilen.
