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Isolamento, caracterização e alto Throughput análise de fluxo extracelular de Mouse primária Renal Tubular as células epiteliais
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells
DOI:

09:40 min

June 20, 2018

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Chapters

  • 00:04Title
  • 00:50Preparing Collagen I Plates
  • 01:31Perfusion, Digestion and Harvesting Kidneys from Mice
  • 02:36Tissue Processing and Primary Tubular Cells Isolation
  • 04:11Mitochondria Bioenergetics Assay
  • 07:26Results: Renal Tubular Epithelial Cell (TEC) Morphology and Metabolism
  • 09:04Conclusion

Summary

Automatic Translation

Este protocolo fornece uma abordagem econômica para isolar e caracterizar pilhas do rato primária renal tubular que podem posteriormente ser sub cultivadas para avaliar funções biológicas renal ex vivo, incluindo bioenergética mitocondrial.

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