/

/

小鼠脊髓损伤模型中内源性神经干细胞活化的干细胞检测

JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Neuroscience

A Neurosphere Assay to Evaluate Endogenous Neural Stem Cell Activation in a Mouse Model of Minimal Spinal Cord Injury

Please note that all translations are automatically generated. Click here for the English version.

小鼠脊髓损伤模型中内源性神经干细胞活化的干细胞检测

DOI:

10.3791/57727-v

•

09:08 min

•

September 13, 2018

•

Nishanth Lakshman², Wenjun Xu, Cindi M. Morshead^2,3

¹Institute of Medical Science,University of Toronto, ²Department of Surgery,University of Toronto, ³Institute of Biomaterials and Biomedical Engineering,University of Toronto

Chapters

00:04Title
00:45Minimal Spinal Cord Injury (SCI) Surgery
04:01Spinal Cord Isolation and Neurosphere Culture
07:18Results: Representative Neurosphere Analyses Following Minimal SCI
08:24Conclusion

Summary

Automatic Translation

在这里, 我们展示了一个最小的脊髓损伤模型的表现, 在一只成年小鼠, 备用的中心运河利基住房内源性神经干细胞。我们展示了如何使用干细胞检测来量化的确定性和原始神经干细胞的活化和迁移后损伤。

Tags

Keywords: Neural Stem Cells Spinal Cord Injury Neurosphere Assay Endogenous Neural Precursor Cells Regenerative Medicine Mouse Model Surgical Technique

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

从胚胎脊髓连合神经的解剖和文化

成年鼠神经干细胞的分离和扩大使用神经干含量

建立小鼠胚胎神经干细胞培养神经球含量

神经细胞集落形成细胞分析检测，以区分善意的神经祖细胞的神经干细胞

侧向液压冲击：创伤性脑损伤小鼠模型

从小鼠胚胎神经管的神经嵴细胞的分离和培养

一个<em>离体</em>激光诱导脊髓损伤模型，以评估轴索变性的机制在实时

胸腺1-gmcmp6 转基因小鼠在原代体血源皮质中的成像神经活性

拉米内切手术和脊髓窗口植入小鼠

神经干细胞向大鼠和小鼠大脑的动脉内传递：对脑缺血的应用

Read Article