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成年斑马鱼模型中霍乱弧菌定植与腹泻的量化研究
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model
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成年斑马鱼模型中霍乱弧菌定植与腹泻的量化研究

DOI:
10.3791/57767-v

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08:03 min

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July 12, 2018

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, , ,
1Department of Microbiology, Immunology, and Biochemistry,Wayne State University School of Medicine

Chapters

  • 00:04Title
  • 00:40Inoculating Zebrafish with V. cholerae
  • 01:49Collecting Intestines and Assessing Bacterial Colonization
  • 04:27Metrics for Fish Diarrhea: Mucin Levels, Protein Levels, A600 and Excreted Bacteria
  • 06:43Results: Colonization and Diarrhea, 18 h After a 6 h Infection Period
  • 07:35Conclusion

Summary

Automatic Translation

Automatic Translation

斑马鱼是一种天然的霍乱弧菌宿主, 可以用来重述和研究整个感染周期从殖民化到传播。在这里, 我们演示如何评估霍乱弧菌的殖民水平和量化腹泻的斑马鱼。

Tags

Vibrio CholeraeZebrafish ModelColonizationDiarrheaIntestinal MicrobiotaLife CycleInfectionDiagnosticDissectionIntestinal Tract

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