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1Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine,University of Freiburg, 2Department of Medicine,Monash University, 3Department of Medicine I, Lighthouse Core Facility, Medical Center,University of Freiburg, 4Department of Cardiology, Augustinerinnen Hospital, Academic Teaching Hospital,University of Cologne
Chapters
- 00:04Title
- 01:01Reverse Transcription
- 01:50Droplet Generation and Digital PCR
- 03:10Droplet Reading and Analyzing
- 03:37Results: miR-499 a Possible Biomarker to Differentiate Patients with CAD and a STEMI
- 04:10Conclusion
MicroRNA circolanti hanno indicato la promessa come biomarcatori per malattie cardiovascolari e infarti miocardici acuti. In questo studio, descriviamo un protocollo per l'estrazione di miRNA, trascrizione d'inversione e digitali PCR per la quantificazione assoluta dei miRNA nel siero dei pazienti con la malattia cardiovascolare.
