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数字 PCR 在急性心肌梗死和心血管疾病中量化循环 MicroRNAs 的临床观察
JoVE Journal
Medicine
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JoVE Journal Medicine
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
DOI:

04:41 min

July 03, 2018

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Chapters

  • 00:04Title
  • 01:01Reverse Transcription
  • 01:50Droplet Generation and Digital PCR
  • 03:10Droplet Reading and Analyzing
  • 03:37Results: miR-499 a Possible Biomarker to Differentiate Patients with CAD and a STEMI
  • 04:10Conclusion

Summary

Automatic Translation

循环 microRNAs 表明, 作为心血管疾病和急性心肌梗死的标志物的承诺。在本研究中, 我们描述了一个 miRNA 提取, 反向转录和数字 PCR 的协议, 以绝对量化的 miRNAs 在心血管疾病患者的血清。

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