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1Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine,University of Freiburg, 2Department of Medicine,Monash University, 3Department of Medicine I, Lighthouse Core Facility, Medical Center,University of Freiburg, 4Department of Cardiology, Augustinerinnen Hospital, Academic Teaching Hospital,University of Cologne
Chapters
- 00:04Title
- 01:01Reverse Transcription
- 01:50Droplet Generation and Digital PCR
- 03:10Droplet Reading and Analyzing
- 03:37Results: miR-499 a Possible Biomarker to Differentiate Patients with CAD and a STEMI
- 04:10Conclusion
MicroRNAs circulantes têm mostrado promessa como biomarcadores para doenças cardiovasculares e aguda do miocárdio. Neste estudo, descrevemos um protocolo para extração de miRNA, a transcrição reversa e PCR digital para a quantificação absoluta dos miRNAs no soro de pacientes com doença cardiovascular.
