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Journal
/
Biochemistry
/
萝卜
品种
多糖的 PNGase 研究
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Analysis of
N
-glycans from
Raphanus sativus
Cultivars Using PNGase H
+
Please note that all translations are automatically generated.
Click here for the English version.
萝卜
品种
多糖的 PNGase 研究
DOI:
10.3791/57979-v
•
08:26 min
•
June 25, 2018
•
Ya-Min Du
,
Shen-Li Zheng
,
Li Liu
,
Josef Voglmeir
,
Gabriel Yedid
1
Glycomics and Glycan Bioengineering Research Center (GGBRC), College of Food Science and Technology
,
Nanjing Agricultural University
,
2
College of Life Science
,
Nanjing Agricultural University
Chapters
00:04
Title
00:41
Isolation of Protein from Radish and Preparation of N-Glycans
02:35
Purification and Fluorescence Derivatization of N-Glycans
04:47
HILIC-UPLC profiling of N-Glycans and MALDI-TOF MS Analysis
06:24
Results: N-glycan Analysis
06:54
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了一种简便、快速的方法, 对不同品种萝卜 (
萝卜
) 的
n-
多糖进行了制备和分析。
Tags
N-glycans
Raphanus Sativus
PNGase H+
Plant Glycoscience
Protein Isolation
TCA Precipitation
N-glycan Release
Solid-phase Extraction
Fluorescence Derivatization
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