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Analyse av N- glykaner fra Raphanus sativus kultivarer bruker PNGase H+
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
DOI:

08:26 min

June 25, 2018

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Chapters

  • 00:04Title
  • 00:41Isolation of Protein from Radish and Preparation of N-Glycans
  • 02:35Purification and Fluorescence Derivatization of N-Glycans
  • 04:47HILIC-UPLC profiling of N-Glycans and MALDI-TOF MS Analysis
  • 06:24Results: N-glycan Analysis
  • 06:54Conclusion

Summary

Automatic Translation

Vi beskriver en enkel og rask metode og analyse av N- glykaner fra forskjellige kultivarer av reddik (Raphanus sativus).

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