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Journal
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Neuroscience
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小鼠原代多元体的
体外
模拟?
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Modeling Charcot-Marie-Tooth Disease
In Vitro
by Transfecting Mouse Primary Motoneurons
Please note that all translations are automatically generated.
Click here for the English version.
小鼠原代多元体的
体外
模拟?
DOI:
10.3791/57988-v
•
07:43 min
•
January 07, 2019
•
Arnaud Jacquier
2
,
Valérie Risson
,
Laurent Schaeffer
3
1
Institut NeuroMyoGène, CNRS UMR5310, INSERM U1217
,
Université Lyon
,
2
Unité Fonctionnelle de Neurogénétique Moléculaire
,
Hospices Civils de Lyon
,
3
Centre de Biotechnologie Cellulaire
,
Hospices Civils de Lyon
Chapters
00:04
Title
00:36
Dissection
01:49
Spinal Cord Cell Suspension
02:58
Motoneuron Enrichment by Gradient Density
04:42
Motoneuron Culture and Magnetofection
06:00
Results: Confocal Images of Magnetofected Motoneurons
06:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这项技术的目的是准备一种高度丰富的来自小鼠脊髓的原发运动神经元 (mns) 培养。为了评估引起 mn 疾病的突变的后果, 我们在这里描述了这些孤立的 m2 的隔离和它们通过磁光的转染。
Tags
Charcot-Marie-Tooth Disease
In Vitro Modeling
Primary Motor Neurons
Transfection
Neurobiology
Motor Neuron Polarity
Axon Guidance
Axon Trafficking
Neurodegenerative Mechanisms
Enriched Motor Neuron Culture
Knockdown Protein
Spinal Cord Dissection
Enzymatic Digestion
Cell Suspension
Density Gradient Centrifugation
Motoneuron Enrichment
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