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甲苯胺蓝染色树脂嵌段评价周围神经形态学
JoVE Journal
Neuroscience
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Neuroscience
Toluidine Blue Staining of Resin-Embedded Sections for Evaluation of Peripheral Nerve Morphology
Please note that all translations are automatically generated.
Click here for the English version.
甲苯胺蓝染色树脂嵌段评价周围神经形态学
DOI:
10.3791/58031-v
•
08:17 min
•
July 03, 2018
•
Adel B. Ghnenis
,
Richard E. Czaikowski
,
Zhaojie J. Zhang
,
Jared S. Bushman
1
School of Pharmacy
,
University of Wyoming
,
2
Department of Zoology and Physiology
,
University of Wyoming
Chapters
00:04
Title
00:36
Surgery and
In Vivo
Nerve Fixation
02:32
Osmium Tetroxide Treatment and Resin Embedding
04:38
Sectioning by Ultramicrotome
05:58
Toluidine Blue Staining
06:47
Results: Transverse Section Images of a Rat Sciatic Nerve Stained with Toluidine Blue
07:48
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出了一个通过获取和染色1-2 µm 段与甲苯胺蓝可视化周围神经精细结构的协议
Tags
Toluidine Blue Staining
Resin-embedded Sections
Peripheral Nerve Morphology
Nerve Injury
Nerve Regeneration
Sciatic Nerve
Nerve Fixation
Nerve Segmentation
Osmium Tetroxide
Epoxy Embedding
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