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Journal
/
Neuroscience
/
哺乳动物轴突再生: 成年小鼠背根神经节
体内
电穿孔的研究
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Investigating Mammalian Axon Regeneration:
In Vivo
Electroporation of Adult Mouse Dorsal Root Ganglion
Please note that all translations are automatically generated.
Click here for the English version.
哺乳动物轴突再生: 成年小鼠背根神经节
体内
电穿孔的研究
DOI:
10.3791/58171-v
•
06:17 min
•
September 01, 2018
•
Qiao Li
,
Cheng Qian
,
Feng-Quan Zhou
2
1
Department of Orthopaedic Surgery
,
Johns Hopkins University School of Medicine
,
2
The Solomon H. Snyder Department of Neuroscience
,
Johns Hopkins University School of Medicine
Chapters
00:04
Title
00:38
Surgical Exposure of the L4 and L5 DRGs
01:37
DRG Injection and Electroporation
02:23
Sciatic Nerve Crush
03:07
DRG and Sciatic Nerve Harvest and Imaging
04:23
Results: EGFP-expressing Axons in the Sciatic Nerve and Spinal Cord
05:35
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
电穿孔是一种有效的方法, 将兴趣基因传递给细胞。本文应用这种方法
对成年小鼠背根神经节的神经元进行了研究, 并对其
在体内
的轴突再生模型进行了描述。
Tags
In Vivo Electroporation
Adult Mouse Dorsal Root Ganglion
Gene Transfection
Axon Regeneration
Sensory Neurons
Sciatic Nerve Crush
Nerve Injury
Gene Overexpression
Gene Knockdown
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