Journal
/
Neuroscience
/
Behandlende pattedyr Axon regenerering: In Vivo elektroporation af voksen mus dorsalrods Ganglion
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Investigating Mammalian Axon Regeneration: In Vivo Electroporation of Adult Mouse Dorsal Root Ganglion

Behandlende pattedyr Axon regenerering: In Vivo elektroporation af voksen mus dorsalrods Ganglion

DOI:
10.3791/58171-v

•

06:17 min

•

September 01, 2018

•

, ,
1Department of Orthopaedic Surgery,Johns Hopkins University School of Medicine, 2The Solomon H. Snyder Department of Neuroscience,Johns Hopkins University School of Medicine

Chapters

  • 00:04Title
  • 00:38Surgical Exposure of the L4 and L5 DRGs
  • 01:37DRG Injection and Electroporation
  • 02:23Sciatic Nerve Crush
  • 03:07DRG and Sciatic Nerve Harvest and Imaging
  • 04:23Results: EGFP-expressing Axons in the Sciatic Nerve and Spinal Cord
  • 05:35Conclusion

Summary

Automatic Translation

Automatic Translation

Elektroporation er en effektiv metode til at levere gener af interesser i celler. Ved at anvende denne fremgangsmåde i vivo på neuroner af voksen mus dorsalrods ganglion (DRG), beskriver vi en model for at studere axon regenerering in vivo.

Tags

Keywords: In Vivo ElectroporationAdult Mouse Dorsal Root GanglionGene TransfectionAxon RegenerationSensory NeuronsSciatic Nerve CrushNerve InjuryGene OverexpressionGene Knockdown

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/58171?article_type=v

Transfection of Mouse Retinal Ganglion Cells by in vivo Electroporation
check_url/58171?article_type=v

Genetisk Undersøgelse af Axon Regeneration med dyrkede voksne dorsale rodganglie neuroner
check_url/58171?article_type=v

In vitro Modellering af Kræft Neural Invasion: Dorsal rodganglie Model
check_url/58171?article_type=v

En tilgang til Forøge Justering og myelination af Dorsal rodganglieneuroner
check_url/58171?article_type=v

Dorsalrodsganglion Injektion og Dorsal Root Crush Skade som en model for Sanse Axon Regeneration
check_url/58171?article_type=v

Laminotomy for lumbal dorsalrods Ganglion adgang og injektion i svin
check_url/58171?article_type=v

Analyse af immunceller i enkelt ischiadicus nerver og dorsalrods Ganglion fra et enkelt museklik bruge flowcytometri
check_url/58171?article_type=v

Hurtig isolering af dorsale Rodganglion makrofager
check_url/58171?article_type=v

In Situ Visualisering af axonvækst og vækstkegledynamik i akutte ex vivo embryonale hjerneskivekulturer
check_url/58171?article_type=v

Berigelse af voksne musedorsalrodganglier neuronkulturer ved immunpanorering

Read Article