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Hyaluronsyre baseret Hydrogels til 3-dimensionelle kultur af Patient-afledte glioblastom celler
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Bioengineering
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JoVE Journal Bioengineering
Hyaluronic-Acid Based Hydrogels for 3-Dimensional Culture of Patient-Derived Glioblastoma Cells

Hyaluronsyre baseret Hydrogels til 3-dimensionelle kultur af Patient-afledte glioblastom celler

DOI:
10.3791/58176-v

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08:35 min

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August 24, 2018

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, , ,
1Department of Bioengineering,University of California, Los Angeles, 2Department of Bioengineering, Jonsson Comprehensive Cancer Center, Broad Stem Cell Research Center, Brain Research Institute,University of California, Los Angeles

Chapters

  • 00:04Title
  • 00:31Hydrogel Crosslinking and Cell Encapsulation
  • 03:11Hydrogel Culture Single Cell Extraction for Flow Cytometry and Cryopreservation for Sectioning
  • 06:10Results: Hyaluronic Acid-Based 3D Patient-Derived Glioblastoma Cell Hydrogel Cultures
  • 07:55Conclusion

Summary

Automatic Translation

Automatic Translation

Vi præsenterer her, en protokol for tre-dimensionelle kultur af patient-afledte glioblastom celler i retvinklet afstemmelige biomaterialer designet til at efterligne den hjerne matrix. Denne fremgangsmåde giver en in vitro-, eksperimentelle platform, der vedligeholder mange egenskaber i vivo glioblastom celler typisk tabt i kulturen.

Tags

Hyaluronic AcidHydrogels3D Cell CultureGlioblastomaExtracellular MatrixPatient-derived CellsCell EncapsulationPEG-MalSilicone Rubber MoldsCell DissociationCell SuspensionGelation

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