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Chapters
- 00:04Title
- 00:34O9-1 Cell Culture
- 01:00Recovery of O9-1 Cells
- 02:59Passage of O9-1 Cells
- 04:27Freezing of O9-1 Cells
- 05:48Manipulation of O9-1 Cells and Differentiation of O9-1 Cells
- 07:11Results: Yap-null Cells Fail to Differentiate into SMA-positive Smooth Muscle Cells
- 07:49Conclusion
O9-1 er en Multipotente mus neurale crest cellelinje. Her beskriver vi detaljerede trinvise protokoller for dyrkning O9-1 celler, differentiere O9-1 celler til specifikke celletyper og genetisk manipulere O9-1 celler ved hjælp af siRNA-medieret knockdown eller CRISPR-Cas9 genom-redigering.
