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定量自动放射法测定维沃脑蛋白合成区域速率
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Neuroscience
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JoVE Journal Neuroscience
Quantitative Autoradiographic Method for Determination of Regional Rates of Cerebral Protein Synthesis In Vivo
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定量自动放射法测定维沃脑蛋白合成区域速率

DOI:
10.3791/58503-v

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11:01 min

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June 28, 2019

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, , , ,
1Section on Neuroadaptation and Protein Metabolism, National Institute of Mental Health,National Institutes of Health

Chapters

  • 00:04Title
  • 00:43Femoral Vein and Artery Catheterization
  • 04:46Tracer Administration and Collection of Blood Samples
  • 06:07Analysis of Plasma Samples to Determine Concentrations of Leucine and L-[1-14C]leucine
  • 07:07Quantitative Autoradiography
  • 07:58Image Analysis and Computation of Regional Rates of Cerebral Protein Synthesis (rCPS)
  • 09:02Results: Effects of Anisomycin on Rates of Cerebral Protein Synthesis
  • 09:57Conclusion

Summary

Automatic Translation

Automatic Translation

蛋白质合成是细胞的关键生物过程。在大脑中,它是自适应变化所必需的。测量完整大脑中蛋白质合成率需要仔细的方法学考虑。这里我们介绍了L-[1-14C]-亮细胞定量自射方法,用于测定体内脑蛋白合成的区域速率。

Tags

Quantitative Autoradiographic MethodRegional RatesCerebral Protein SynthesisIn VivoBrain Protein SynthesisQuantitativeAwake Behaving AnimalAutoradiographic TechniqueFemoral ArteryFemoral VeinSurgical ProcedureSutureCatheterHeparinized Saline

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