JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Neuroscience
/
複数の齧歯動物の頭脳の同時セクショニング
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Neuroscience
Simultaneous Cryosectioning of Multiple Rodent Brains
Please note that all translations are automatically generated.
Click here for the English version.
複数の齧歯動物の頭脳の同時セクショニング
DOI:
10.3791/58513-v
•
06:37 min
•
September 18, 2018
•
Tabitha R.F. Green
2,3
,
J. Bryce Ortiz
,
Jordan L. Harrison
,
Jonathan Lifshitz
3,4
,
Rachel K. Rowe
3,4
1
Department of Child Health
,
University of Arizona College of Medicine
,
2
Department of Biological Sciences
,
University of Bath
,
3
BARROW Neurological Institute at Phoenix Children’s Hospital
,
4
Phoenix Veteran Affairs Healthcare System
,
5
Department of Basic Medical Sciences
,
University of Arizona College of Medicine
Chapters
00:04
Title
00:44
Cryoprotection of Perfused Brains and Brain Freezing
03:18
Preparing the Megabrain for Sectioning on the Cryostat
04:14
Cryosectioning the Megabrain
05:24
Results: Freezing and Sectioning Brain Tissue from Multiple Animals with Megabrain
05:54
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
ここでは、凍結し、単一脳の処理時間を節約する代わりとして複数の動物から脳組織をセクション プロトコルを提案します。これは免疫組織化学的に染色変動を減少し、時間セクショニングとイメージングを減少します。
Tags
Simultaneous Cryosectioning
Multiple Rodent Brains
Immunohistochemistry
Time-saving Method
Cryosection
Embedding Mold
Isopentane
Optimal Cutting Temperature
OCT Compound
Transcardial Perfusion
Paraformaldehyde
Sucrose Solution
Cerebellum
Olfactory Bulbs
Brain Hemispheres
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
齧歯類の脳における同時fMRIと電気生理学
全体の開発、
ショウジョウバエ
の脳の
ex vivoで
培養
同時脳波、リアルタイム乳酸濃度の測定と齧歯類大脳皮質ニューロン活動のOptogeneticマニピュレーション
同時電気生理学的記録およびげっ歯類の脳における阻害剤のマイクロ注射
胚性齧歯類の脳から単離、プライマリ脳ドーパミンニューロンの文化と長期メンテナンス
早期の生後早期のマウス脳に介在ニューロン前駆体の Homochronic 移植
キイロショウジョウバエ
幼虫と大人の脳の代謝解析
マウス脳のフリーフローティング免疫染色
複数の小生物からの自動マルチモーダル刺激と同時神経記録
マウス新生児脳のマイクロCTイメージングと形態解析
Read Article